Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging. / Bjorknes, Benedicte; Neye, Oliver Emil; Hamerlik, Petra; Jauffred, Liselotte.

In: PLoS ONE, Vol. 18, No. 2, 0281161, 09.02.2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bjorknes, B, Neye, OE, Hamerlik, P & Jauffred, L 2023, 'Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging', PLoS ONE, vol. 18, no. 2, 0281161. https://doi.org/10.1371/journal.pone.0281161

APA

Bjorknes, B., Neye, O. E., Hamerlik, P., & Jauffred, L. (2023). Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging. PLoS ONE, 18(2), [0281161]. https://doi.org/10.1371/journal.pone.0281161

Vancouver

Bjorknes B, Neye OE, Hamerlik P, Jauffred L. Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging. PLoS ONE. 2023 Feb 9;18(2). 0281161. https://doi.org/10.1371/journal.pone.0281161

Author

Bjorknes, Benedicte ; Neye, Oliver Emil ; Hamerlik, Petra ; Jauffred, Liselotte. / Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging. In: PLoS ONE. 2023 ; Vol. 18, No. 2.

Bibtex

@article{57adec5cdcb74f4dbc062f97147e91e0,
title = "Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging",
abstract = "Glioblastoma tumors form in brains' white matter and are fast-growing and aggressive. Poor prognosis is the result of therapeutic resistance and infiltrating growth into the surrounding brain. Here we present a protocol for the detection of the cytoskeleton intermediate filament, vimentin, in cells at the proliferating spheroid surface. By combining a classical invasion assay with immunofluorescence and light-sheet imaging, we find that it is exactly these cytoskeleton-reinforcing cells on the spheroid's surface that will start the infiltration. We anticipate our results to be the starting point of more sophisticated investigation of anti-cancer drug effects on cytoskeleton reorganisation.",
keywords = "TO-MESENCHYMAL TRANSITION, INTERMEDIATE-FILAMENTS, VIMENTIN, INVASION",
author = "Benedicte Bjorknes and Neye, {Oliver Emil} and Petra Hamerlik and Liselotte Jauffred",
year = "2023",
month = feb,
day = "9",
doi = "10.1371/journal.pone.0281161",
language = "English",
volume = "18",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging

AU - Bjorknes, Benedicte

AU - Neye, Oliver Emil

AU - Hamerlik, Petra

AU - Jauffred, Liselotte

PY - 2023/2/9

Y1 - 2023/2/9

N2 - Glioblastoma tumors form in brains' white matter and are fast-growing and aggressive. Poor prognosis is the result of therapeutic resistance and infiltrating growth into the surrounding brain. Here we present a protocol for the detection of the cytoskeleton intermediate filament, vimentin, in cells at the proliferating spheroid surface. By combining a classical invasion assay with immunofluorescence and light-sheet imaging, we find that it is exactly these cytoskeleton-reinforcing cells on the spheroid's surface that will start the infiltration. We anticipate our results to be the starting point of more sophisticated investigation of anti-cancer drug effects on cytoskeleton reorganisation.

AB - Glioblastoma tumors form in brains' white matter and are fast-growing and aggressive. Poor prognosis is the result of therapeutic resistance and infiltrating growth into the surrounding brain. Here we present a protocol for the detection of the cytoskeleton intermediate filament, vimentin, in cells at the proliferating spheroid surface. By combining a classical invasion assay with immunofluorescence and light-sheet imaging, we find that it is exactly these cytoskeleton-reinforcing cells on the spheroid's surface that will start the infiltration. We anticipate our results to be the starting point of more sophisticated investigation of anti-cancer drug effects on cytoskeleton reorganisation.

KW - TO-MESENCHYMAL TRANSITION

KW - INTERMEDIATE-FILAMENTS

KW - VIMENTIN

KW - INVASION

U2 - 10.1371/journal.pone.0281161

DO - 10.1371/journal.pone.0281161

M3 - Journal article

C2 - 36757917

VL - 18

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 2

M1 - 0281161

ER -

ID: 338299327