Raman spectra of DL-Alanine crystals were investigated in the 50-3200cm^-1 spectral region for temperatures ranging from 15 to 295 K. The crystalline structure of DL-Alanine represents a rare example of an amino acid racemate crystallizing in a non-centrosymmetric space group. From this study, we have observed changes in the wavenumber of modes associated with both rocking of CO₂^- and skeletal vibrations. On the other hand, neither changes in the modes associated to CH or CH₃ vibrations nor substantial modifications of the lattice modes of the crystal were observed. Such result indicates slight changes of the CO₂ group orientation without observation of a solid-solid phase transition in the DL-Alanine crystal.