Size Effect of TiO2 Nanoparticles as Food Additive and Potential Toxicity
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Size Effect of TiO2 Nanoparticles as Food Additive and Potential Toxicity. / Lu, Naiyan; Chen, Zhe; Song, Jiaoyang; Weng, Yuyan; Yang, Guofeng; Liu, Qingrun; Yang, Kai; Lu, Xuemei; Liu, Yu.
In: Food Biophysics, Vol. 17, 04.11.2021, p. 75-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Size Effect of TiO2 Nanoparticles as Food Additive and Potential Toxicity
AU - Lu, Naiyan
AU - Chen, Zhe
AU - Song, Jiaoyang
AU - Weng, Yuyan
AU - Yang, Guofeng
AU - Liu, Qingrun
AU - Yang, Kai
AU - Lu, Xuemei
AU - Liu, Yu
PY - 2021/11/4
Y1 - 2021/11/4
N2 - Titanium dioxide (TiO2), as a representative of metallic oxides, is widely used as food additive to serve as coloring or antibacterial packaging fortifier. However, in recent years, the potential hazards of nano-sized TiO2 food additives to human beings is still in doubt. In this work, we systematically studied the interactions between three sizes of TiO2 particles and the cell membrane systems with confocal laser scanning microscopy. We used both giant unilamellar vesicles and human colon cancer cells to mimic actual cell membrane systems. The results demonstrate the strong effect of particle size on membrane disruption and leakage behaviors. Among the three sizes of TiO2 nanoparticles (NPs) used in this work, the TiO2 NPs with the size of 110-300 nm are safe. In contrast, NPs with the size of 10-30 nm and 50-100 nm will cause the rupture and leakage of cell membranes. These indicates that the cytotoxicity of nano-sized TiO2 particles increases with the decrease of particle sizes. In addition, it is found that the size effect of TiO2 particles further depends on the membrane charge state. Simulations were also introduced to understand the results. This study would provide a theoretical basis for the production designing and safety standard of metallic oxide food additives.
AB - Titanium dioxide (TiO2), as a representative of metallic oxides, is widely used as food additive to serve as coloring or antibacterial packaging fortifier. However, in recent years, the potential hazards of nano-sized TiO2 food additives to human beings is still in doubt. In this work, we systematically studied the interactions between three sizes of TiO2 particles and the cell membrane systems with confocal laser scanning microscopy. We used both giant unilamellar vesicles and human colon cancer cells to mimic actual cell membrane systems. The results demonstrate the strong effect of particle size on membrane disruption and leakage behaviors. Among the three sizes of TiO2 nanoparticles (NPs) used in this work, the TiO2 NPs with the size of 110-300 nm are safe. In contrast, NPs with the size of 10-30 nm and 50-100 nm will cause the rupture and leakage of cell membranes. These indicates that the cytotoxicity of nano-sized TiO2 particles increases with the decrease of particle sizes. In addition, it is found that the size effect of TiO2 particles further depends on the membrane charge state. Simulations were also introduced to understand the results. This study would provide a theoretical basis for the production designing and safety standard of metallic oxide food additives.
KW - Titanium dioxide
KW - Size effect
KW - Giant unilamellar vesicles
KW - Phospholipid membrane
KW - Membrane interruption and leakage
KW - TITANIUM-DIOXIDE
KW - PARTICLES
KW - AG
U2 - 10.1007/s11483-021-09695-7
DO - 10.1007/s11483-021-09695-7
M3 - Journal article
VL - 17
SP - 75
EP - 83
JO - Food Biophysics
JF - Food Biophysics
SN - 1557-1858
ER -
ID: 284400356