Effect of ergosterol on the interlamellar spacing of deuterated yeast phospholipid multilayers
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Effect of ergosterol on the interlamellar spacing of deuterated yeast phospholipid multilayers. / Luchini, Alessandra; Delhom, Robin; Cristiglio, Viviana; Knecht, Wolfgang; Wacklin-Knecht, Hanna; Fragneto, Giovanna.
In: Chemistry and Physics of Lipids, Vol. 227, 104873, 03.2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of ergosterol on the interlamellar spacing of deuterated yeast phospholipid multilayers
AU - Luchini, Alessandra
AU - Delhom, Robin
AU - Cristiglio, Viviana
AU - Knecht, Wolfgang
AU - Wacklin-Knecht, Hanna
AU - Fragneto, Giovanna
N1 - Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2020/3
Y1 - 2020/3
N2 - Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked to function. Ergosterol is the main sterol molecule found in the cell membranes of yeasts and other fungi. Like the cholesterol found in mammalian cells, ergosterol has been proposed to have an ordering and condensing effect on saturated phospholipid membranes. The effects of cholesterol have been investigated extensively and result in an increase in the membrane thickness and the lipid acyl chain order. Less information is available on the effects of ergosterol on phospholipid membranes. Neutron Diffraction (ND) was used to characterize the effect of ergosterol on lipid multilayers prepared with deuterated natural phospholipids extracted from the yeast Pichia pastoris. The data show that the effect of ergosterol on membranes prepared from the natural phospholipid extract rich in unsaturated acyl chains, differs from what has been observed previously in membranes rich in saturated phospholipids. In contrast to cholesterol in synthetic phospholipid membranes, the presence of ergosterol up to 30 mol % in yeast phospholipid membranes only slightly altered the multilayer structure. In particular, only a small decrease in the multilayer d-spacing was observed as function of increasing ergosterol concentrations. This result highlights the need for further investigation to elucidate the effects of ergosterol in biological lipid mixtures.
AB - Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked to function. Ergosterol is the main sterol molecule found in the cell membranes of yeasts and other fungi. Like the cholesterol found in mammalian cells, ergosterol has been proposed to have an ordering and condensing effect on saturated phospholipid membranes. The effects of cholesterol have been investigated extensively and result in an increase in the membrane thickness and the lipid acyl chain order. Less information is available on the effects of ergosterol on phospholipid membranes. Neutron Diffraction (ND) was used to characterize the effect of ergosterol on lipid multilayers prepared with deuterated natural phospholipids extracted from the yeast Pichia pastoris. The data show that the effect of ergosterol on membranes prepared from the natural phospholipid extract rich in unsaturated acyl chains, differs from what has been observed previously in membranes rich in saturated phospholipids. In contrast to cholesterol in synthetic phospholipid membranes, the presence of ergosterol up to 30 mol % in yeast phospholipid membranes only slightly altered the multilayer structure. In particular, only a small decrease in the multilayer d-spacing was observed as function of increasing ergosterol concentrations. This result highlights the need for further investigation to elucidate the effects of ergosterol in biological lipid mixtures.
U2 - 10.1016/j.chemphyslip.2020.104873
DO - 10.1016/j.chemphyslip.2020.104873
M3 - Journal article
C2 - 31926858
VL - 227
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
M1 - 104873
ER -
ID: 243195330