The dynamics of phage predation on a microcolony

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

The dynamics of phage predation on a microcolony. / Eriksen, Rasmus Skytte; Larsen, Frej; Svenningsen, Sine Lo; Sneppen, Kim; Mitarai, Namiko.

I: Biophysical Journal, Bind 123, Nr. 2, 2024, s. 147-156.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Eriksen, RS, Larsen, F, Svenningsen, SL, Sneppen, K & Mitarai, N 2024, 'The dynamics of phage predation on a microcolony', Biophysical Journal, bind 123, nr. 2, s. 147-156. https://doi.org/10.1016/j.bpj.2023.12.003

APA

Eriksen, R. S., Larsen, F., Svenningsen, S. L., Sneppen, K., & Mitarai, N. (2024). The dynamics of phage predation on a microcolony. Biophysical Journal, 123(2), 147-156. https://doi.org/10.1016/j.bpj.2023.12.003

Vancouver

Eriksen RS, Larsen F, Svenningsen SL, Sneppen K, Mitarai N. The dynamics of phage predation on a microcolony. Biophysical Journal. 2024;123(2):147-156. https://doi.org/10.1016/j.bpj.2023.12.003

Author

Eriksen, Rasmus Skytte ; Larsen, Frej ; Svenningsen, Sine Lo ; Sneppen, Kim ; Mitarai, Namiko. / The dynamics of phage predation on a microcolony. I: Biophysical Journal. 2024 ; Bind 123, Nr. 2. s. 147-156.

Bibtex

@article{d818374250f941489d257a8a1e9c2947,

title = "The dynamics of phage predation on a microcolony",

abstract = "Phage predation is an important factor for controlling the bacterial biomass. At face value, dense microbial habitats are expected to be vulnerable to phage epidemics due to the abundance of fresh hosts immediately next to any infected bacteria. Despite this, the bacterial microcolony is a common habitat for bacteria in nature. Here, we experimentally quantify the fate of microcolonies of Escherichia coli exposed to virulent phage T4. It has been proposed that the outer bacterial layers of the colony will shield the inner layers from the phage invasion and thereby constrain the phage to the colony's surface. We develop a dynamical model that incorporates this shielding mechanism and fit the results with experimental measurements to extract important phage-bacteria interaction parameters. The analysis suggests that, while the shielding mechanism delays phage attack, T4 phage are able to diffuse so deep into the dense bacterial environment that colony-level survival of the bacterial community is challenged.",

author = "Eriksen, {Rasmus Skytte} and Frej Larsen and Svenningsen, {Sine Lo} and Kim Sneppen and Namiko Mitarai",

note = "Publisher Copyright: {\textcopyright} 2023 Biophysical Society",

year = "2024",

doi = "10.1016/j.bpj.2023.12.003",

language = "English",

volume = "123",

pages = "147--156",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Cell Press",

number = "2",

}

RIS

TY - JOUR

T1 - The dynamics of phage predation on a microcolony

AU - Eriksen, Rasmus Skytte

AU - Larsen, Frej

AU - Svenningsen, Sine Lo

AU - Sneppen, Kim

AU - Mitarai, Namiko

PY - 2024

Y1 - 2024

N2 - Phage predation is an important factor for controlling the bacterial biomass. At face value, dense microbial habitats are expected to be vulnerable to phage epidemics due to the abundance of fresh hosts immediately next to any infected bacteria. Despite this, the bacterial microcolony is a common habitat for bacteria in nature. Here, we experimentally quantify the fate of microcolonies of Escherichia coli exposed to virulent phage T4. It has been proposed that the outer bacterial layers of the colony will shield the inner layers from the phage invasion and thereby constrain the phage to the colony's surface. We develop a dynamical model that incorporates this shielding mechanism and fit the results with experimental measurements to extract important phage-bacteria interaction parameters. The analysis suggests that, while the shielding mechanism delays phage attack, T4 phage are able to diffuse so deep into the dense bacterial environment that colony-level survival of the bacterial community is challenged.

AB - Phage predation is an important factor for controlling the bacterial biomass. At face value, dense microbial habitats are expected to be vulnerable to phage epidemics due to the abundance of fresh hosts immediately next to any infected bacteria. Despite this, the bacterial microcolony is a common habitat for bacteria in nature. Here, we experimentally quantify the fate of microcolonies of Escherichia coli exposed to virulent phage T4. It has been proposed that the outer bacterial layers of the colony will shield the inner layers from the phage invasion and thereby constrain the phage to the colony's surface. We develop a dynamical model that incorporates this shielding mechanism and fit the results with experimental measurements to extract important phage-bacteria interaction parameters. The analysis suggests that, while the shielding mechanism delays phage attack, T4 phage are able to diffuse so deep into the dense bacterial environment that colony-level survival of the bacterial community is challenged.

U2 - 10.1016/j.bpj.2023.12.003

DO - 10.1016/j.bpj.2023.12.003

M3 - Journal article

C2 - 38069473

AN - SCOPUS:85180281334

VL - 123

SP - 147

EP - 156

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 2

ER -

ID: 382900458

Niels Bohr Institutet