TY - JOUR

T1 - Label-free optical interferometric microscopy to characterize morphodynamics in living plants

AU - Ebrahimi, Samira

AU - Moreno-Pescador, Guillermo

AU - Persson, Staffan

AU - Jauffred, Liselotte

AU - Bendix, Poul Martin

PY - 2023

Y1 - 2023

N2 - During the last century, fluorescence microscopy has played a pivotal role in a range of scientific discoveries. The success of fluorescence microscopy has prevailed despite several shortcomings like measurement time, photobleaching, temporal resolution, and specific sample preparation. To bypass these obstacles, label-free interferometric methods have been developed. Interferometry exploits the full wavefront information of laser light after interaction with biological material to yield interference patterns that contain information about structure and activity. Here, we review recent studies in interferometric imaging of plant cells and tissues, using techniques such as biospeckle imaging, optical coherence tomography, and digital holography. These methods enable quantification of cell morphology and dynamic intracellular measurements over extended periods of time. Recent investigations have showcased the potential of interferometric techniques for precise identification of seed viability and germination, plant diseases, plant growth and cell texture, intracellular activity and cytoplasmic transport. We envision that further developments of these label-free approaches, will allow for high-resolution, dynamic imaging of plants and their organelles, ranging in scales from sub-cellular to tissue and from milliseconds to hours.

AB - During the last century, fluorescence microscopy has played a pivotal role in a range of scientific discoveries. The success of fluorescence microscopy has prevailed despite several shortcomings like measurement time, photobleaching, temporal resolution, and specific sample preparation. To bypass these obstacles, label-free interferometric methods have been developed. Interferometry exploits the full wavefront information of laser light after interaction with biological material to yield interference patterns that contain information about structure and activity. Here, we review recent studies in interferometric imaging of plant cells and tissues, using techniques such as biospeckle imaging, optical coherence tomography, and digital holography. These methods enable quantification of cell morphology and dynamic intracellular measurements over extended periods of time. Recent investigations have showcased the potential of interferometric techniques for precise identification of seed viability and germination, plant diseases, plant growth and cell texture, intracellular activity and cytoplasmic transport. We envision that further developments of these label-free approaches, will allow for high-resolution, dynamic imaging of plants and their organelles, ranging in scales from sub-cellular to tissue and from milliseconds to hours.

KW - digital holographic cell imaging

KW - optical coherence tomography

KW - label-free microscopy

KW - material transport

KW - intereferometric imaging

KW - plant cells and tissues

KW - speckle imaging

KW - plant morphodynamics

KW - DIGITAL HOLOGRAPHIC MICROSCOPY

KW - COHERENCE TOMOGRAPHY

KW - BIOSPECKLE ACTIVITY

KW - ROOT-GROWTH

KW - REVEALS

KW - DIFFRACTION

KW - DYNAMICS

KW - LEAF

KW - TOOL

U2 - 10.3389/fpls.2023.1156478

DO - 10.3389/fpls.2023.1156478

M3 - Review

C2 - 37284726

VL - 14

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1156478

ER -