Enhanced DNA repair through droplet formation and p53 oscillations

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Enhanced DNA repair through droplet formation and p53 oscillations. / Heltberg, Mathias S.; Lucchetti, Alessandra; Hsieh, Feng-Shu; Duy Pham Minh Nguyen; Chen, Sheng-hong; Jensen, Mogens H.

In: Cell, Vol. 185, No. 23, 10.11.2022, p. 4394-4408.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Heltberg, MS, Lucchetti, A, Hsieh, F-S, Duy Pham Minh Nguyen, Chen, S & Jensen, MH 2022, 'Enhanced DNA repair through droplet formation and p53 oscillations', Cell, vol. 185, no. 23, pp. 4394-4408. https://doi.org/10.1016/j.cell.2022.10.004

APA

Heltberg, M. S., Lucchetti, A., Hsieh, F-S., Duy Pham Minh Nguyen, Chen, S., & Jensen, M. H. (2022). Enhanced DNA repair through droplet formation and p53 oscillations. Cell, 185(23), 4394-4408. https://doi.org/10.1016/j.cell.2022.10.004

Vancouver

Heltberg MS, Lucchetti A, Hsieh F-S, Duy Pham Minh Nguyen, Chen S, Jensen MH. Enhanced DNA repair through droplet formation and p53 oscillations. Cell. 2022 Nov 10;185(23):4394-4408. https://doi.org/10.1016/j.cell.2022.10.004

Author

Heltberg, Mathias S. ; Lucchetti, Alessandra ; Hsieh, Feng-Shu ; Duy Pham Minh Nguyen ; Chen, Sheng-hong ; Jensen, Mogens H. / Enhanced DNA repair through droplet formation and p53 oscillations. In: Cell. 2022 ; Vol. 185, No. 23. pp. 4394-4408.

Bibtex

@article{265f384f768341f68cb3d7ddf326dccc,
title = "Enhanced DNA repair through droplet formation and p53 oscillations",
abstract = "Living organisms are constantly exposed to DNA damage, and optimal repair is therefore crucial. A charac-teristic hallmark of the response is the formation of sub-compartments around the site of damage, known as foci. Following multiple DNA breaks, the transcription factor p53 exhibits oscillations in its nuclear concen-tration, but how this dynamics can affect the repair remains unknown. Here, we formulate a theory for foci formation through droplet condensation and discover how oscillations in p53, with its specific periodicity and amplitude, optimize the repair process by preventing Ostwald ripening and distributing protein material in space and time. Based on the theory predictions, we reveal experimentally that the oscillatory dynamics of p53 does enhance the repair efficiency. These results connect the dynamical signaling of p53 with the micro-scopic repair process and create a new paradigm for the interplay of complex dynamics and phase transi-tions in biology.",
author = "Heltberg, {Mathias S.} and Alessandra Lucchetti and Feng-Shu Hsieh and {Duy Pham Minh Nguyen} and Sheng-hong Chen and Jensen, {Mogens H.}",
year = "2022",
month = nov,
day = "10",
doi = "10.1016/j.cell.2022.10.004",
language = "English",
volume = "185",
pages = "4394--4408",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "23",

}

RIS

TY - JOUR

T1 - Enhanced DNA repair through droplet formation and p53 oscillations

AU - Heltberg, Mathias S.

AU - Lucchetti, Alessandra

AU - Hsieh, Feng-Shu

AU - Duy Pham Minh Nguyen, null

AU - Chen, Sheng-hong

AU - Jensen, Mogens H.

PY - 2022/11/10

Y1 - 2022/11/10

N2 - Living organisms are constantly exposed to DNA damage, and optimal repair is therefore crucial. A charac-teristic hallmark of the response is the formation of sub-compartments around the site of damage, known as foci. Following multiple DNA breaks, the transcription factor p53 exhibits oscillations in its nuclear concen-tration, but how this dynamics can affect the repair remains unknown. Here, we formulate a theory for foci formation through droplet condensation and discover how oscillations in p53, with its specific periodicity and amplitude, optimize the repair process by preventing Ostwald ripening and distributing protein material in space and time. Based on the theory predictions, we reveal experimentally that the oscillatory dynamics of p53 does enhance the repair efficiency. These results connect the dynamical signaling of p53 with the micro-scopic repair process and create a new paradigm for the interplay of complex dynamics and phase transi-tions in biology.

AB - Living organisms are constantly exposed to DNA damage, and optimal repair is therefore crucial. A charac-teristic hallmark of the response is the formation of sub-compartments around the site of damage, known as foci. Following multiple DNA breaks, the transcription factor p53 exhibits oscillations in its nuclear concen-tration, but how this dynamics can affect the repair remains unknown. Here, we formulate a theory for foci formation through droplet condensation and discover how oscillations in p53, with its specific periodicity and amplitude, optimize the repair process by preventing Ostwald ripening and distributing protein material in space and time. Based on the theory predictions, we reveal experimentally that the oscillatory dynamics of p53 does enhance the repair efficiency. These results connect the dynamical signaling of p53 with the micro-scopic repair process and create a new paradigm for the interplay of complex dynamics and phase transi-tions in biology.

U2 - 10.1016/j.cell.2022.10.004

DO - 10.1016/j.cell.2022.10.004

M3 - Journal article

C2 - 36368307

VL - 185

SP - 4394

EP - 4408

JO - Cell

JF - Cell

SN - 0092-8674

IS - 23

ER -

ID: 337352354