Single-cell tracking reveals super-spreading brain cancer cells with high persistence

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Standard

Single-cell tracking reveals super-spreading brain cancer cells with high persistence. / Nousi, Aimilia; Sogaard, Maria Tangen; Audion, Melanie; Jauffred, Liselotte.

I: Biochemistry and Biophysics Reports, Bind 28, 101120, 01.12.2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nousi, A, Sogaard, MT, Audion, M & Jauffred, L 2021, 'Single-cell tracking reveals super-spreading brain cancer cells with high persistence', Biochemistry and Biophysics Reports, bind 28, 101120. https://doi.org/10.1016/j.bbrep.2021.101120

APA

Nousi, A., Sogaard, M. T., Audion, M., & Jauffred, L. (2021). Single-cell tracking reveals super-spreading brain cancer cells with high persistence. Biochemistry and Biophysics Reports, 28, [101120]. https://doi.org/10.1016/j.bbrep.2021.101120

Vancouver

Nousi A, Sogaard MT, Audion M, Jauffred L. Single-cell tracking reveals super-spreading brain cancer cells with high persistence. Biochemistry and Biophysics Reports. 2021 dec. 1;28. 101120. https://doi.org/10.1016/j.bbrep.2021.101120

Author

Nousi, Aimilia ; Sogaard, Maria Tangen ; Audion, Melanie ; Jauffred, Liselotte. / Single-cell tracking reveals super-spreading brain cancer cells with high persistence. I: Biochemistry and Biophysics Reports. 2021 ; Bind 28.

Bibtex

@article{53f61976dbe8409f917610690de92b69,
title = "Single-cell tracking reveals super-spreading brain cancer cells with high persistence",
abstract = "Cell migration is a fundamental characteristic of vital processes such as tissue morphogenesis, wound healing and immune cell homing to lymph nodes and inflamed or infected sites. Therefore, various brain defect diseases, chronic inflammatory diseases as well as tumor formation and metastasis are associated with aberrant or absent cell migration. We embedded multicellular brain cancer spheroids in Matrigel (TM) and utilized single-particle tracking to extract the paths of cells migrating away from the spheroids. We found that - in contrast to local invasion - single cell migration is independent of Matrigel (TM) concentration and is characterized by high directionality and persistence. Furthermore, we identified a subpopulation of super-spreading cells with >200-fold longer persistence times than the majority of cells. These results highlight yet another aspect of cell heterogeneity in tumors.",
keywords = "Invasion assay, Glioblastoma, Single-cell tracking, Diffusion models, BASEMENT-MEMBRANE MATRIX, RANDOM MOTION, ANOMALOUS DIFFUSION, STOCHASTIC-MODEL, TUMOR SPHEROIDS, MIGRATION, MOTILITY, TRANSITION, DYNAMICS, GROWTH",
author = "Aimilia Nousi and Sogaard, {Maria Tangen} and Melanie Audion and Liselotte Jauffred",
year = "2021",
month = dec,
day = "1",
doi = "10.1016/j.bbrep.2021.101120",
language = "English",
volume = "28",
journal = "Biochemistry and Biophysics Reports",
issn = "2405-5808",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Single-cell tracking reveals super-spreading brain cancer cells with high persistence

AU - Nousi, Aimilia

AU - Sogaard, Maria Tangen

AU - Audion, Melanie

AU - Jauffred, Liselotte

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Cell migration is a fundamental characteristic of vital processes such as tissue morphogenesis, wound healing and immune cell homing to lymph nodes and inflamed or infected sites. Therefore, various brain defect diseases, chronic inflammatory diseases as well as tumor formation and metastasis are associated with aberrant or absent cell migration. We embedded multicellular brain cancer spheroids in Matrigel (TM) and utilized single-particle tracking to extract the paths of cells migrating away from the spheroids. We found that - in contrast to local invasion - single cell migration is independent of Matrigel (TM) concentration and is characterized by high directionality and persistence. Furthermore, we identified a subpopulation of super-spreading cells with >200-fold longer persistence times than the majority of cells. These results highlight yet another aspect of cell heterogeneity in tumors.

AB - Cell migration is a fundamental characteristic of vital processes such as tissue morphogenesis, wound healing and immune cell homing to lymph nodes and inflamed or infected sites. Therefore, various brain defect diseases, chronic inflammatory diseases as well as tumor formation and metastasis are associated with aberrant or absent cell migration. We embedded multicellular brain cancer spheroids in Matrigel (TM) and utilized single-particle tracking to extract the paths of cells migrating away from the spheroids. We found that - in contrast to local invasion - single cell migration is independent of Matrigel (TM) concentration and is characterized by high directionality and persistence. Furthermore, we identified a subpopulation of super-spreading cells with >200-fold longer persistence times than the majority of cells. These results highlight yet another aspect of cell heterogeneity in tumors.

KW - Invasion assay

KW - Glioblastoma

KW - Single-cell tracking

KW - Diffusion models

KW - BASEMENT-MEMBRANE MATRIX

KW - RANDOM MOTION

KW - ANOMALOUS DIFFUSION

KW - STOCHASTIC-MODEL

KW - TUMOR SPHEROIDS

KW - MIGRATION

KW - MOTILITY

KW - TRANSITION

KW - DYNAMICS

KW - GROWTH

U2 - 10.1016/j.bbrep.2021.101120

DO - 10.1016/j.bbrep.2021.101120

M3 - Journal article

C2 - 34541340

VL - 28

JO - Biochemistry and Biophysics Reports

JF - Biochemistry and Biophysics Reports

SN - 2405-5808

M1 - 101120

ER -

ID: 289234640