Fabian Czerwinski – Niels Bohr Institute - University of Copenhagen

Niels Bohr Institute > Research > PhD theses > 2010 > Fabian Czerwinski


Fabian Czerwinski

Title: Allan Variance Analysis and Fast Camera Detection Schemes for Optical Tweezers

A thesis submitted September 27, 2010 for the degree of Doctor of Philosophy and defended November 11, 2010.

Bio Complexity at the Niels Bohr Institute, The Graduate School of Science, Faculty of Science University of Copenhagen Denmark.

Academic Advisor:
Lene B. Oddershede

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Abstract

Allan Variance Analysis and Fast Camera Detection Schemes for Optical Tweezers

Optical tweezers are an ubiquitous tool in modern biophysics. By introducing Allan variance analysis complementary to power spectral analysis, we improved the most prominent calibration protocols by reducing the number of assumptions. We employed this method to find the optimal calibration interval, to optimize acquisition parameters, and to quantify low frequency noise.

Going further, we developed a camera-based detection scheme that allowed for stable and reliable real-time acquisition of optical tweezers experiments. We present data and discuss how this scheme as well as Allan variance analysis hold potential for other single-molecule techniques.

Both developments pave the road to establish optical tweezers as a diagnostic tool for the investigation of potentially harmful interactions of fullerenes and DNA. To understand this interaction, we applied different single-molecule approaches and put them into perspective to one another.

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