Talk by Dr. Aldo Jesorka – Niels Bohr Institute - University of Copenhagen

Niels Bohr Institute > Calendar > 2012 > Talk by Dr. Aldo Jesorka

Talk by Dr. Aldo Jesorka

Hydrodynamically confined flow devices represent a powerful approach to localized solution delivery within a liquid environment that is miscible with the solution to be applied [1]. The greatest strength of the technology is the highly localized exposure, which allows for contamination-free superfusion of micrometer sized surface areas or surface-associated objects, such as single adherent cells. We have developed an free-standing microfluidic pipette made in poly(dimethyl siloxane) having a circulating liquid tip that generates a self-confining volume, i.e., a virtual flow chamber, in front of the outlet channels [2]. This non-contact, open-volume microfluidic device serves as a solution handling and dispensing tool, which can address individual cells in culture dishes or tissue slices. On-chip integration of solution reservoirs and microfluidic functionality, including mixing, and switching circuitry, have resulted in a multifaceted research instrument, which has already demonstrated potential in cell biology and preclinical research [3,4]. The device concept, fabrication strategies, and application examples will be presented.

[1] Hydrodynamic Flow Confinement Technology in Microfluidic Perfusion Devices. A. Ainla, G. D. M. Jeffries, and A. Jesorka; Micromachines, Special Issue: Microflow Controllers 3(2), 442-461, 2012. [2] A Microfluidic Pipette for Single-Cell Pharmacology. A. Ainla, E.T. Jansson, N. Stepanyants, O. Orwar, and A. Jesorka; Anal. Chem. 82 (11), 4529 - 4536, 2010. [3] A Multifunctional Pipette. A. Ainla, G. D. M. Jeffries, R. Brune, O. Orwar and A. Jesorka; Lab Chip 12, 1255-1261, 2012. [4] Single-Cell Electroporation Using a Multifunctional Pipette. A. Ainla, S. Xu, N. Sanchez, G.D.M. Jeffries, and A. Jesorka, Lab Chip 2012, DOI:10.1039.

Talk by Dr. Aldo Jesorka, Ass. Professor. Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden