Dynamic buckling of actin within filopodia

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Dynamic buckling of actin within filopodia. / Leijnse, Natascha; Oddershede, Lene B; Bendix, Pól Martin.

I: Communicative & Integrative Biology, Bind 8, Nr. 2, 21.10.2015, s. e1022010.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Leijnse, N, Oddershede, LB & Bendix, PM 2015, 'Dynamic buckling of actin within filopodia', Communicative & Integrative Biology, bind 8, nr. 2, s. e1022010. https://doi.org/10.1080/19420889.2015.1022010

APA

Leijnse, N., Oddershede, L. B., & Bendix, P. M. (2015). Dynamic buckling of actin within filopodia. Communicative & Integrative Biology, 8(2), e1022010. https://doi.org/10.1080/19420889.2015.1022010

Vancouver

Leijnse N, Oddershede LB, Bendix PM. Dynamic buckling of actin within filopodia. Communicative & Integrative Biology. 2015 okt. 21;8(2):e1022010. https://doi.org/10.1080/19420889.2015.1022010

Author

Leijnse, Natascha ; Oddershede, Lene B ; Bendix, Pól Martin. / Dynamic buckling of actin within filopodia. I: Communicative & Integrative Biology. 2015 ; Bind 8, Nr. 2. s. e1022010.

Bibtex

@article{bdc39574098e455a822c196b0cc371cf,
title = "Dynamic buckling of actin within filopodia",
abstract = "Filopodia are active tubular structures protruding from the cell surface which allow the cell to sense and interact with the surrounding environment through repetitive elongation-retraction cycles. The mechanical behavior of filopodia has been studied by measuring the traction forces exerted on external substrates.(1) These studies have revealed that internal actin flow can transduce a force across the cell surface through transmembrane linkers like integrins. In addition to the elongation-retraction behavior filopodia also exhibit a buckling and rotational behavior. Filopodial buckling in conjunction with rotation enables the cell to explore a much larger 3-dimensional space and allows for more complex, and possibly stronger, interactions with the external environment.(2) Here we focus on how bending of the filopodial actin dynamically correlates with pulling on an optically trapped microsphere which acts like an external substrate attached to the filopodial tip. There is a clear correlation between presence of actin near the tip and exertion of a traction force, thus demonstrating that the traction force is transduced along the actin shaft inside the filopodium. By extending a filopodium and holding it while measuring the cellular response, we also monitor and analyze the waiting times for the first buckle observed in the fluorescently labeled actin shaft.",
author = "Natascha Leijnse and Oddershede, {Lene B} and Bendix, {P{\'o}l Martin}",
year = "2015",
month = oct,
day = "21",
doi = "10.1080/19420889.2015.1022010",
language = "English",
volume = "8",
pages = "e1022010",
journal = "Communicative & Integrative Biology",
issn = "1942-0889",
publisher = "Taylor & Francis",
number = "2",

}

RIS

TY - JOUR

T1 - Dynamic buckling of actin within filopodia

AU - Leijnse, Natascha

AU - Oddershede, Lene B

AU - Bendix, Pól Martin

PY - 2015/10/21

Y1 - 2015/10/21

N2 - Filopodia are active tubular structures protruding from the cell surface which allow the cell to sense and interact with the surrounding environment through repetitive elongation-retraction cycles. The mechanical behavior of filopodia has been studied by measuring the traction forces exerted on external substrates.(1) These studies have revealed that internal actin flow can transduce a force across the cell surface through transmembrane linkers like integrins. In addition to the elongation-retraction behavior filopodia also exhibit a buckling and rotational behavior. Filopodial buckling in conjunction with rotation enables the cell to explore a much larger 3-dimensional space and allows for more complex, and possibly stronger, interactions with the external environment.(2) Here we focus on how bending of the filopodial actin dynamically correlates with pulling on an optically trapped microsphere which acts like an external substrate attached to the filopodial tip. There is a clear correlation between presence of actin near the tip and exertion of a traction force, thus demonstrating that the traction force is transduced along the actin shaft inside the filopodium. By extending a filopodium and holding it while measuring the cellular response, we also monitor and analyze the waiting times for the first buckle observed in the fluorescently labeled actin shaft.

AB - Filopodia are active tubular structures protruding from the cell surface which allow the cell to sense and interact with the surrounding environment through repetitive elongation-retraction cycles. The mechanical behavior of filopodia has been studied by measuring the traction forces exerted on external substrates.(1) These studies have revealed that internal actin flow can transduce a force across the cell surface through transmembrane linkers like integrins. In addition to the elongation-retraction behavior filopodia also exhibit a buckling and rotational behavior. Filopodial buckling in conjunction with rotation enables the cell to explore a much larger 3-dimensional space and allows for more complex, and possibly stronger, interactions with the external environment.(2) Here we focus on how bending of the filopodial actin dynamically correlates with pulling on an optically trapped microsphere which acts like an external substrate attached to the filopodial tip. There is a clear correlation between presence of actin near the tip and exertion of a traction force, thus demonstrating that the traction force is transduced along the actin shaft inside the filopodium. By extending a filopodium and holding it while measuring the cellular response, we also monitor and analyze the waiting times for the first buckle observed in the fluorescently labeled actin shaft.

U2 - 10.1080/19420889.2015.1022010

DO - 10.1080/19420889.2015.1022010

M3 - Journal article

C2 - 26479403

VL - 8

SP - e1022010

JO - Communicative & Integrative Biology

JF - Communicative & Integrative Biology

SN - 1942-0889

IS - 2

ER -

ID: 153446905